ESTIMATING OF RAPD MARKER ASSOCIATED TO COLOR GENE IN Zinnia Elegans JACQ

Flower color contributes mainly to the market value of ornamental plants including Zinnia elegans Jacq. The coloration of flowers is determined by several genes. The previous study showed that Zinnia coloration is controlled by
two independent genes, which are white color as dominant (chromogen) and its suppressor. The aim of this study is to
investigate marker that associated with chromogen based on RAPD. DNA from each of 240 individuals in M2 population
was extracted from leaf by CTAB method (Doyle and Doyle, 1990). Fifteen primers were used to amplify DNA fragments.
The PCR program was consisted of preheating at 94°C for 1 min, followed by 45 cycles of denaturation at 94°C for 30 sec, annealing at 38°C for 30 sec, and elongation at 72°C for 1 min 30 sec. The last cycle was followed by a final extension cycle at 72°C for 7 min. Six among fifteen primers were selected and produced 60 loci which is 100% was polymorphic. The binary data were analyzed using Fisher Exact Test and Kendall Tau correlation in the SAS program to get correlation between markers and those traits. The result showed OPB171400 and OPA181500 markers were associated with chromogen in Zinnia.