Antibacterial Activity Analysis of Zanthoxylum Acanthopodium DC Extract on Bacteria of Bacillus Subtilis, and Salmonella Typhi

Research has been conducted on the antibacterial activity analysis of Zanthoxylum acanthopodium DC extract on Bacillus subtilis, Salmonella typhi using disc diffusion method. This study aims to determine the ability of Zanthoxylum acanthopodium DC extract in inhibiting the growth of Bacillus subtillis, and Salmonella typhi. Zanthoxylum acanthopodium DC was extracted by maceration method using 96% ethanol. In this extraction process, phytochemical test was carried out to find out the secondary metabolite content in Zanthoxylum acanthopodium DC. Phytochemical test indicate Zanthoxylum acanthopodium DC contained alkaloids, tannins, saponins, and steroids. Antibacterial activity analysis were measured in three concentrations of 25%, 50%, 75%. The best antibacterial activity was observed on Salmonella typhi bacteria with 75% extract concentration which produced a clear zone diameter of 19 mm and 20 mm.