In vitro Growth of CattleyaspOrchid from Leaf Explants with Growth Regulators

Harahap, Fauziyah and Hariyadi, Itra and Silitonga, Melva and Suriani, Cicik and Edi, Syahmi and Ningsih, Ayu Putri (2023) In vitro Growth of CattleyaspOrchid from Leaf Explants with Growth Regulators. Jurnal Pembelajaran Dan Biologi Nukleus, 9 (1). pp. 192-200. ISSN p-ISSN: 2442-9481; e-ISSN: 2685-7332

Full text not available from this repository.

Abstract

Using leaf explants, this research aimed at identifying the type of growth response that can be created and the optimal mix of media for the growth of Cattleya sp orchid. This was an experimental investigation employing the RAL method (completely randomized design) with a combination of 2.4-D (0;1; 2; 3 ppm) and Kinetin (0;0.3;0.6 ppm) repeated three times. According to the results of this study, the growth regulators 2.4-D and kinetin were unable to promote the development of all explants. Explants transferred to media containing polyvinylpyrrolidone (PVP) to prevent browning after 12 weeks of observation. Then the results of the study also found the emergence of shoots in the treatment P0 (control), P2 (2,4-D 0 + Kinetin 0.6 ppm), P8 (2,4-D 2 + Kinetin 0.6) and P11 (2.4- D 3 + K 0.6 ppm) in the 3rd week of observation, the appearance of callus at P5 (2.4-D 1 + Kinetin 0.6 ppm) in the 4th week of observation

Item Type: Article
Keywords: Cattleya sp; Growth Regulators; 2.4 Dichlorophenoxy Acetic Acid; Kinetin
Subjects: Q Science > QH Natural history > QH301 Biology
S Agriculture > SB Plant culture
S Agriculture > SB Plant culture > SB128 Growth regulators
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam
Depositing User: Mrs Catur Dedek Khadijah
Date Deposited: 01 May 2023 12:12
Last Modified: 01 May 2023 12:13
URI: https://digilib.unimed.ac.id/id/eprint/51974

Actions (login required)

View Item
View Item