DNA BARCODING OF ZINGIBER LOERZINGII VALETON USING RIBULOSE-1,5-BIPHOSPHATE CARBOXYLASE-OXYGENASE LARGE SUBUNIT GENE (RBCL) GENE LOCUS

Rahmayani, Ladiez and Lazuardi and Harahap, Fauziyah and Manalu, Kartika and Idami, Zahratul and Prasetya, Eko (2021) DNA BARCODING OF ZINGIBER LOERZINGII VALETON USING RIBULOSE-1,5-BIPHOSPHATE CARBOXYLASE-OXYGENASE LARGE SUBUNIT GENE (RBCL) GENE LOCUS. Jurnal Biosains Unimed, 7 (3). pp. 166-173. ISSN 2460-6804; 2443-1230

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Abstract

DNA barcode is one of the molecular techniques used to identify and classify living things. Z. loerzingii is currently reported as a rare and endemic plants that is only found in North Sumatra. Scientific studies for Z. Loerzingii is measly done so that the information about this plant is limited while a clear identity on a plant is essential to discover the potential. This study aims to determine the molecular characteristics of Z. loerzingii by using DNA barcodes and assessing the phylogenetic relationship based on the rbcL gene locus. DNA was isolated with a commercial kit. The rbcL gene locus in the chloroplast genome of Z. loerzingii amplified using the Polymerase Chain Reaction technique to produce amplicon with the length approximately 600 bp. Consensus sequence merging generate a sequence with 576 bp length. The phylogenetic tree reconstruction was carried out using the Neighbor-Joining method and the Kimura-2-Parameter calculation model showed Z. loerzingii included in the monophyletic group with Zingiber mioga and Zingiber officinale as the sister taxa. The results for the molecular diversity analysis of Z. Loerzingii point out that in all samples of Z. loerzingii which collected from Cagar Alam Sibolangit have no molecular or genetic diversity. Therefore, it can be concluded that DNA barcoding with the rbcL gene locus can be used as a method to identify Z. loerzingii molecularly and efficient in determining their phylogenetic with other species.

Item Type: Article
Keywords: DNA Barcoding; Zingiber loerzingii; rbcL
Subjects: Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH301 Biology > QH705 Economic biology
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam
Depositing User: Mrs Catur Dedek Khadijah
Date Deposited: 01 May 2023 11:56
Last Modified: 01 May 2023 11:56
URI: https://digilib.unimed.ac.id/id/eprint/51972

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