MOLECULAR CHARACTERIZATION OF ENDOPHY MUSHROOMS AS ANTI-MICROBIAL FROM BLUME LEAVES (PREMNA PUBESCENS. BUAS-BUAS)

Alternative utilization of natural resources through the use of endophytic microbes that live in every tissue in plants. Endophytic microbes can live in plant tissues in the form of bacteria or fungi. Endophytic fungi have the same ability as their host plants in producing secondary metabolite compounds. Plants that contain secondary metabolites and are known to be able to inhibit the growth of pathogenic bacteria are Blume (savage) plants.
Blume tree (Premna pubescens, Buas-buas) is a plant that contains antibacterial because it contains secondary metabolites and saponins, flavonoids including luteolin and apigenin which have a good effect on preventing bacterial growth. Endophytic fungi found in a plant do not all have the ability to inhibit bacterial growth. So, a study was conducted to determine which endophytic fungi have the ability as antibacterial. Furthermore, endophytic fungi will be identified molecularly to ascertain the types and species of fungi and bacteria that have potential as antibacterial.
The method in this research is to produce endophytic fungi that have the potential as antibacterial by first isolating the endophytic fungi present on Blume (buas-buas) leaves. All pure isolates of endophytic fungi found during the isolation process of Blume (buas-buas) leaves were tested for their antibacterial activity against the test bacteria. The pure isolates of endophytic fungi will then be identified molecularly by extracting DNA from the pure isolates of endophytic fungi which have the potential as antibacterial.
Based on the results of the tests carried out both on endophytic bacteria against pathogenic bacteria or endophytic fungi against pathogenic bacteria, the results obtained proved that Blume (savage) leaves contain substances such as flavonoids, alkaloids, phenolics, and saponins. Blume extract (buas-buas) is known to have antimicrobial ability to inhibit microbial growth in inhibiting the growth of pathogenic microbes.