De Novo Transcriptome Profiling of Buasbuas (Premna pubescens. Blume)

Diningrat, Diky Setya (2018) De Novo Transcriptome Profiling of Buasbuas (Premna pubescens. Blume). Biotechnology, 17 (2). pp. 76-85. ISSN 1682-2978; ISSN 1682-296X

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Abstract

Buasbuas is the one of the medicinal plants in Indonesia that contains bioactive compounds potential as antimicrobial, antioxidant, antidiabetes, antiinflammation and anticancer. Exploring the pathway and gene related of buasbuas bioactive compounds production has led to the renaissance of understanding buasbuas molecular mechanism database. The aim of this study was to developed data-mining framework of buasbuas to study plant specialized metabolism for phytochemical biosynthesis. Material and Methods: This project was started by collecting shoots and leaves of Buasbuas. Focus of the project was exploring the molecular mechanisms on biosynthesis phytochemical of Buasbuas. Illumina Mi-Seq Next Generation Sequencing was utilized to understand the molecular mechanisms of biosynthesis. Transcriptomes then trimmed and assembled with CLCBio genomic software. Assembled contigs then annotated towards Arabidopsis thaliana using CLC Bio genomic software. Digital Gene Expression was performed to analyze the transcriptional changes in control culture and treatment. Results: There were 5.342 unigenes that expressed only in treatment shoot cultures. Annotation with Gene Ontology showed that 57.9% (3.446) unigenes play role in Biological Process, 56.7% (3.375) unigenes play role in Cellular Components and 63.4% (3.772) unigenes play role in Molecular Functions. Annotation with Kyoto Encyclopedia of Genes and Genomes shows 853 unigenes essentially have role in 24 biological pathways. Highest process with highest unigenes involvement is biosynthesis of plant hormones and biosynthesis of alkaloids. Conclusion: This study showed that phytochemical biosynthesis in buasbuas induces level expression of several genes involved in the jasmonic acid, cytokinin, gibberellin, salicylic acid and ethylene biosynthesis pathway.

Item Type: Article
Keywords: Buasbuas; bioactive compounds; transcriptome; phytochemicals; biosynthetic pathway
Subjects: Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH301 Biology > QH540 Ecology
Q Science > QH Natural history > QH301 Biology > QH705 Economic biology
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: Mrs Catur Dedek Khadijah
Date Deposited: 25 Oct 2021 07:03
Last Modified: 25 Oct 2021 07:03
URI: https://digilib.unimed.ac.id/id/eprint/43487

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