PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY Coix lacryma-jobi OIL

Coix lacryma-jobi (Hanjeli) is known to posses
anti-microbial properties. Therefore, phytochemical compounds
of C. lacryma-jobi have been studied to produce novel antimicrobial agents as treatments against antibiotic-resistant
bacteria.The objective of this study was to determine the
phytochemical composition and antibacterial activity of the C.
lacryma-jobi oil against Escherichia coli, Staphylococcus
aureus, and Bacillus subtilis. The phytochemical composition
of the oil was determined via gas chromatography mass spectrophotometry (GC-MS). Moreover, agar disk and agar well
diffusion were employed to screen the antibacterial activity of
the oil. An agar well diffusion test was implemented to
determinate MIC’s (minimum inhibitory concentrations).
Dodecanoic acid, tetradecanoic acid, 2,3-dihydroxypropylester,
1,3-dioctanoin, N-methoxy-N-methyl-3,4-dihydro-2H-thiopyran6-carboxamide, propanamide, 5-Amino-1-(quinolin-8-yl)-1,2,3-
triazole-4-carboxamide, and pyridine were identified in the C.
lacryma-jobi oil. The MIC value of the oil was 0.031 g/L and
the MBC of the oil was 0.125 g/L effective in all test bacteria.
Dodecanoic acid displayed inhibitory activity against
gram-positive and gram-negative bacteria. Therefore, our
research demonstrated C. lacryma-jobi (Hanjeli) oil exhibited
antibacterial activity against E. coli, S. aureus, and B. subtilis.
These research suggest that C. lacryma-jobi root oil could be
used for medicinal purposes; however clinical and in vivo tests
must be performed to evaluate its potential as an antibacterial
agent.