Idramsa (2018) Biodegradation process of crude oil and bioemulsifier activity by a bacterial consortium culture. In: The 4th International Conference on Biological Sciences and Biotechnology, 8-9 Desember 2018, Medan, North Sumatera, Indonesia.
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Abstract
This study reports the analysis of biosurfactant production by a bacterial consortium
culture (CC) during biodegradation of crude oil. The ability of CC (5% v/v), grown in
Minimal Medium (MM) added with 1% (v/v) Tapis crude oil, to produce biosurfactant under
different incubation times (0, 2, 4, 6 and 8 week) was investigated. Biosurfactant activity was
estimated by water surface tension changes and emulsification index (E24). Maximum
biosurfactant production was observed at 2 weeks incubation period, after which the yield
gradually decreased. The biosurfactant produced by the CC significantly decreased the surface
tension of water from 72 dynes/cm to 52 dynes/cm (p 0.05) at week 4. Emulsification test
indicated that this biosurfactant effectively emulsified the crude oil with an emulsification
index (E24) of 85% (p 0.05). This is significantly better (p 0.05) when compared to other
chemical surfactants tested namely SDS (53.3%), Tween-20 (50.8%) and Triton X-100 (55%).
These results indicate the capability of CC to produce biosurfactant during crude oil
degradation and this can be exploited for enhanced bioremediation in oil contaminated areas
Item Type: | Conference or Workshop Item (Paper) |
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Keywords: | biology; bacterial consortium culture; crude oil |
Subjects: | Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH301 Biology > QH540 Ecology Q Science > QR Microbiology Q Science > QR Microbiology > QR75 Bacteria |
Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam |
Depositing User: | Mrs Catur Dedek Khadijah |
Date Deposited: | 01 Mar 2021 11:23 |
Last Modified: | 01 Mar 2021 11:23 |
URI: | https://digilib.unimed.ac.id/id/eprint/41378 |