ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF NONPOLAR TO POLAR SOLVENT EXTRACTS OF Sarang Banua (Clerodenrum fragrans Vent Wild) LEAVES

Sarangbanua plant, known as Clerodendrumfragrans Vent Willd, is a division of the Verbanaceae family found in
Simalungun district of Indonesia, which is traditionally used as medicinal plants. The antioxidant and antibacterial activities of the C. fragrans leave extract was prepared by dissolving it in non-polar to polar solvents and treated by DPPH (1,1- diphenyl-2-picrylhydrazin) and diffusion methods. The results showed that the ethanol (polar) extract of the C. fragrans leaves had the highest antioxidant activity than ethyl acetate (semi-polar) and n-hexane (non-polar) extracts, which had IC50 values of 22.37 ppm, 27.26 ppm, and 88.77 ppm respectively. Vitamin C as a positive controlled with the IC50 value of 20.18 ppm. Furthermore, the ethyl acetate (semi-polar) extract of the C. fragrans leaves had the greatest inhibition value on Gram-positive bacteria of S. aureus (90.0%)with inhibitory zones of 11.25 ± 0.2 mm, meanwhile the n-hexane (non-polar) extract had the greatest inhibition against Gram-negative
bacteria of the E.coli(77.21%)with inhibitory zones of 10.5±0.3 mm.