ANTIOXIDANT ACTIVITY OF VACUUM COLUMN CHROMATOGRAPHY FRACTIONS OF ETHANOL EXTRACT OF SARANG BANUA (Clerodenrum fragrans vent wild) LEAVES

Sarang banua plants are found in Simalungun, North Sumatra, Indonesia, which have been used by the community as traditional medicinal plants. The result of plant determination, sarang banua plant is a type of Clerodendrum fragrans Vent Willd, including the family Verbenaceae. The antioxidant activity of vacuum column chromatography fractions of ethanol extract of C. fragrans leaves was completed. This study aims to find a pure fraction through vacuum column chromatography from the ethanol extract of C. fragrans leaves which has antioxidant activity. Purification of ethanol extract of C. Fragrans was carried out using vacuum column chromatography with silica gel 1.07733.1000 stationary phase and n-hexane, ethyl acetate and methanol as mobile phase. The antioxidant activity test of the fractions were carried out by DPPH free radical reduction method. The antioxidant test results using the DPPH free radical scavenging method showed that the F5 fraction had higher antioxidant activity than F4 and F3 fractions with IC50 values in a row 19.80; 24.65 and 84.02 ppm. The IC50 value of vitamin C as a positive control is 22.25 ppm. The F5 fraction has antioxidant activity higher than Vitamin C (positive control) and includes a very strong category of antioxidants.