EFFECT OF IPTG CONCENTRATION ON RECOMBINANT HUMAN PRETHROMBIN-2 EXPRESSION IN Escherichia coli BL21(DE3) ARCTICEXPRESS

Silaban, Saronom and Gaffar, S. and Simorangkir, Murniaty and Maksum, I. P. and Subroto, Toto (2018) EFFECT OF IPTG CONCENTRATION ON RECOMBINANT HUMAN PRETHROMBIN-2 EXPRESSION IN Escherichia coli BL21(DE3) ARCTICEXPRESS. In: The 12th Congress of Indonesian Soc. for Biochemistry and Molecular Biology in Conjunction With The 2nd Int. Conf. "Collaboration Seminar of Chemistry and Industry (CoSCI)" and AnMicro Workshop, 11-12 Oct 2018, Universitas Airlangga.

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Abstract

Production of recombinant proteins in E. coli still facesa bottle neck due to formation of inclusion bodies. The level of gene transcription can be regulated by using appropriate concentration of isopropyl-β-D-thiogalactoside (IPTG) inducer. The purpose of this study is to determine the effect of IPTG concentration on human prethrombin-2 (hPT-2) expression. The hPT-2 expression was induced by various concentrations of IPTG (0.01 mM, 0.025 mM, 0.05 mM, 0.075 mM, 0.1 mM, 0.2 mM and 0.3 mM) at 12°C for 18 hours hosted by E. coli BL21(DE3) ArcticExpress. The result show that the suitable IPTG concentration for induction of hPT-2 in E. coli BL21(DE3) ArcticExpress was 0.1 mM. It was indicated from the 62-kDa protein band obtained from the soluble fraction on SDS-PAGE. It concluded that the IPTG concentration affect the rate of rhPT-2 expression.

Item Type: Conference or Workshop Item (Paper)
Keywords: ArcticExpress; Expression; Prethrombin-2; Escherichia coli
Subjects: Q Science > QD Chemistry > QD450 Physical and theoretical chemistry
Q Science > QD Chemistry > QD71 Analytical chemistry
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia
Depositing User: Mrs Harly Christy Siagian
Date Deposited: 16 Apr 2020 08:22
Last Modified: 16 Apr 2020 08:35
URI: https://digilib.unimed.ac.id/id/eprint/39102

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