PERTUMBUHAN KALUS PADA EKSPLAN BATANG MANGGIS (Garcinia mangostana L. ) YANG DITANAM SECARA IN VITRO

This study was conducted to determine the effect of growth regulators (ZPT) on callus induction of mangosteen (Garcinia mangostana l.) From the source of stems planted in vitro. The research was carried out from November 2017 to June 2018. This study used a research design that is a non factorial completely randomized design with 8 repetitions using 3 types of combination repetition of 0.1 ppm TDZ, 0.1 ppm + 2 TDZ , 4 D 1.5 ppm, TDZ 0.1 ppm + 2.4 D 3ppm The results showed that the administration of growth regulators affected callus growth time at 0.1 ppm + 2.4 D 3 ppm TDZ treatment on the 11th day after induction, from the three treatments the callus texture had a dense texture and callus biomass The best combination of growth regulators in inducing callus texture is found in all 3 combinations. All treatments produce a compact compact textured callus. The best combination of growth regulators in inducing callus
stack height is found in MS + TDZ + 2,4 d 3 treatment. The best treatment in inducing callus surface area was in the 0.1 ppm TDZ treatment, and the best callus weight was produced by 0.1 ppm + 2.4 D 3 TDZ treatment. From these results it can be seen that the induction medium with a
combination of ZPT 3. 0.1 ppm + 2.4 D 3 ppm TDZ is a good combination in inducing callus.