PENGEMBANGAN SENSOR ELEKTROKIMIA UNTUK PENENTUAN UREA

The development of electrochemical sensor for the determination of urea is needed as a strategy to obtain an accurate analytical instrument. It is known that the concentration of urea in the body fluid is a key indicator for some diseases such as kidney problem, coronary heart diseases, and stroke. Therefore, a sensitive analytical instrument for the determination of urea is compulsory in the laboratory for fast and accurate assay. The aim of this research is to develop an electrochemical sensor in a potentiometric method for the determination of urea. The research was carried out by constructing of a sensing device selective for urea through immobilization of enzyme urease in the electrode surface, followed by interfacing of an working electrode with a potentiometer as instrument transducer, and the optimization of the urea sensor. The research results showed that immobilization of urease onto the surface of wolfram (W) wire has successfully done by using of poly vinyl alcohol (PVA) matrix. The sensing device give positive response to urea standard solution. In the first stage, the urea sensor gave linear response lies between 0.3 - 10 mM urea, slope 58.30 mV perdecade urea concentration (r2 = 0.994).