Sihombing, Kawan and Tamba, Monica Chrisdayanti and Marbun, Winry S and Situmorang, Manihar (2018) UREASE IMMOBILIZAD POTENTIOMETRIC BIOSENSOR FOR DETERMINATION UREA. Indian Journal of Chemistry-Section a Inorganic, Physical, Theoretical and Analytical Chemistry, 57A. pp. 175-180. ISSN 0975-0975
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Abstract
A simple potentiometric biosensor for the determination of urea is reported in this work. The biosensor has been constructed by immobilization of urease at tungsten electrode using polyvinyl alcohol matrix followed by the sealing process with plasticised polyvinyl chloride. The sensing device gives fast and sensitive response to urea. A linear calibration curve in the concentration range of 0.01-50 mM urea is obtained with slope 42.7 mV per decade urea concentration (r2 = 0.993), and detection a limit of 0.01 mM urea.
Item Type: | Article |
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Keywords: | Electroanalytical chemistry; Biosensors; Potentiometric biosensors; Enzyme electrode; Tungsten electrode; Immobilized enzyme; Urea |
Subjects: | Q Science > QD Chemistry > QD450 Physical and theoretical chemistry Q Science > QD Chemistry > QD71 Analytical chemistry |
Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
Depositing User: | Mrs Harly Christy Siagian |
Date Deposited: | 19 Apr 2018 10:23 |
Last Modified: | 19 Apr 2018 10:31 |
URI: | https://digilib.unimed.ac.id/id/eprint/29897 |