IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DAN UJI AKTIFITAS ANTIOKSIDAN EKSTRAK METANOL DAUN BOSIBOSI (TIMONIUS FLAVESCENS (JACQ) BAKER, F1 MAURITIUS 1877) DENGAN METODE DPPH

Napitupulu, Ari Agustinus (2015) IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DAN UJI AKTIFITAS ANTIOKSIDAN EKSTRAK METANOL DAUN BOSIBOSI (TIMONIUS FLAVESCENS (JACQ) BAKER, F1 MAURITIUS 1877) DENGAN METODE DPPH. Undergraduate thesis, UNIMED.

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Abstract

Bosibosi (Timonius flavenscens (jacq) Baker) termasuk tumbuhan Rubiaceae. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder dan aktivitas antioksidan dari ekstrak metanol daun bosibosi. Penelitian ini dilaksanakan pada bulan Mei – Agustus 2015 di laboratorium Kimia Bahan Alam, Jurusan Kimia FMIPA Universitas Sumatra Utara. Sampel daun bosibosi yang digunakan dalam penelitian ini berasal dari kota Tarutung, Kabupaten Tapanuli Utara. Metode ekstraksi yang digunakan adalah metode maserasi dengan menggunakan pelarut metanol. Analisis senyawa metabolit sekunder dilakukan dengan metode skrining fitokimia yaitu senyawa alkaloid, flavonoid, terpenoid, steroid, saponin pada ekstrak metanol daun bosibosi. Identifikasi metabolit sekunder dengan pengamatan reaksi warna, pengendapan dan buih. Pengujian aktivitas antioksidan ekstrak metanol daun bosibosi dilakukan dengan metode reduksi senyawa radikal bebas 1,1- Diphenyl-2-Picrylhydrazyl (DPPH). Hasil penelitian menunjukan bahwa golongan senyawa metabolit sekunder yang teridentifikasi dalam ekstrak metanol daun bosibosi adalah Saponin, terpenoid, Flavonoid. Hasil analisis terhadap aktivitas antioksidan menunjukan bahwa nilai IC50 ekstrak metanol daun bosibosi sebesar 22,015 ppm. Hal ini menunjukan bahwa daun bosibosi memiliki potensi sebagai antioksidan.

Item Type: Thesis (Undergraduate)
Contributors:
ContributionNameNIDN/NIDK
Thesis advisorSipahutar, Herbert196106261987101001
Call Number: SK-2016 BIO 003
Keywords: Bosibosi; Maserasi; Skrining Fitokimia; Metabolit Sekunder, DPPH; Antioksidan.
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QM Human anatomy
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: Mr Maknun
Date Deposited: 16 May 2016 03:03
URI: http://digilib.unimed.ac.id/id/eprint/5249

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