OPTIMASI SENYAWA METABOLIT SEKUNDER BAKTERI SIMBION SPONS YANG BERPOTENSI SEBAGAI ANTIBAKTERI

M, INDRA PRIAWAN (2018) OPTIMASI SENYAWA METABOLIT SEKUNDER BAKTERI SIMBION SPONS YANG BERPOTENSI SEBAGAI ANTIBAKTERI. Undergraduate thesis, UNIMED.

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Abstract

Penelitian ini bertujuan untuk optimasi produk metabolit sekunder bakteri simbion spons yang diperoleh dari perairan Pandan Sibolga. Sampel yang digunakan ialah bakteri simbion spons yang diperoleh dari 3 spesies spons. Bakteri simbion spons didapatkan dengan mengisolasi bakteri keatas permukaan media Zobell Marine Agar. Bakteri yang telah tumbuh di karakterisasi berdasarkan karakteristik morfologi koloni (warna, bentuk, elevasi, dan tepian) serta dilakukan identifikasi gram bakteri. Dari hasil karakterisasi diperoleh 13 isolat bakteri simbion spons dengan 5 isolat berasal dari Spons 1 (S1I1,S1I2,S1I3,S1I4, dan S1I5), 4 isolat dari Spons 2 (S2I1,S2I2,S2I3, dan S2I4), dan 4 isolat dari Spons 3 (S3I1, S3I2, S3I3, S3I4). Isolat bakteri simbion spons dilakukan uji aktivitas antibakteri untuk mengetahui isolat yang berpotensi sebagai antibakteri dengan menguji pada 3 spesies bakteri uji yaitu Staphylococcus aureus, Eschericia coli, dan Salmonella typhii. Dari uji yang dilakukan didapatkan 9 isolat yang berpotensi sebagai antibakteri dengan 6 isolat berspektrum luas (S1I1, S1I3, S1I4, S2I1, S2I2, dan S3I2) dan 3 isolat berspektrum sempit (S1I5,S3I1, dan S3I3). Adapun zona hambat terluas terhadap bakteri S.auerus, E.coli, dan S.typhii didapakan pada isolat S3I2,S2I2, dan S3I3 dengan luas zona hambat 11mm, 9mm, dan 11mm. Berdasarkan luas zona hambat dan spektrumnya, maka diperoleh 3 isolat terbaik untuk dilakukan optimasi metabolit sekunder yaitu isolat S1I3, S2I2, dan S3I2. Optimasi waktu didapatkan waktu terbaik untuk produksi metabolit sekunder masing masing isolat adalah S1I3 pada jam inkubasi ke-72 hingga ke-76, isolat S2I2 pada jam inkubasi ke-36 hingga ke-75 jam, dan isolat S3I2 pada jam inkubasi ke-73 hingga ke-76. Adapun pada optimasi media, didapatkan media NB dapat mengoptimasi pertumbuhan isolat S1I3 dan S3I2 serta media M13 mengoptimalkan produksi isolat S2I2.

Item Type: Thesis (Undergraduate)
Contributors:
ContributionNameNIDN/NIDK
Thesis advisorGultom, Endang Sulistiyarini198105152009122004
Call Number: SK-2019 BIO 037
Keywords: Bakteri Simbion Spons, Aktivitas Antibakteri, Optimasi Metabolit Sekunder
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH301 Biology > QH359 Evolution
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: Mr Fifri Juanda Harahap
Date Deposited: 28 Feb 2019 13:56
URI: http://digilib.unimed.ac.id/id/eprint/32673

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