Antibacterial Activity of Endophytic ER-2 Bacterial Isolates from Plant Cotylelobium melanoxylon

Endophytic microbes are microorganisms that colonize the parts of plant
organs and interact with host plant without causing any symptoms or diseases in the host
plant. Endophytic microbial colonization is found in various types of plant organs,
namely: roots, stems, leaves and seeds. This study is focused on endophytic bacteria
mostly conducted intensively on wild plants or forests in the tropical ecosystems.
Various trees or tropical forest plants have potentials as traditional medicine and in
cooperation with the bacteria. The C. melanoxylon has been utilized by local community
as antidiarrheal medicine because of the content of active antibacterial compounds. The
presence of endophytic bacteria in tissues of the stem of C. melanoxylon was observed
the bacteria were directly isolated from the tissues by planting pour plate on Tryptic Soya
Agar (TSA) medium. The antibacterial activity of endophytic bacteria isolates was
examined using a dual cultural assay against Escherichia coli ATCC 35218 and
Staphyllococcus aureus ATCC 25923 as a model bacterial. Morphological, physiological,
and biochemical characters were identified based on the ability of the isolates to grow.
The endophytic bacteria isolate of strain ER-2 was inhibitat the growth of E. coli ATCC
35218 and S. aureus ATCC 25923.