ANALISIS HUBUNGAN KEKERABATAN BAKTERI SIMBIONS SPONS TERKAIT DENGAN SINTESIS SENYAWA BIOAKTIF MENGGUNAKAN PENANDA 16S RNA

Sari, Intan Mayang (2017) ANALISIS HUBUNGAN KEKERABATAN BAKTERI SIMBIONS SPONS TERKAIT DENGAN SINTESIS SENYAWA BIOAKTIF MENGGUNAKAN PENANDA 16S RNA. Undergraduate thesis, UNIMED.

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Abstract

Spons sebagai penghasil senyawa bioaktif seperti antibakteri, antifungal, antitumor dan antivirus. Kemampuan spons dalam menghasilkan senyawa bioaktif dikarenakan hubungan simbiotik dengan mikroorganisme dalam hal ini bakteri yang bersimbion dengan spons. Penelitian ini bertujuan untuk melakukan karakterisasi molekuler isolat bakteri simbion spons berdasarkan sekuen gen 16S RNA. Pada penelitian ini menggunakan 2 isolat bakteri simbion spons yang telah diisolasi DNA hasil isolasi DNA dilanjutkan dengan amplifikasi gen 16S RNA dengan menggunakan mesin amplifikasi PCR (Polymerase Chain Reaction). Hasil dari amplifikasi dilanjutkan dengan sequensing gen 16S RNA. Hasil dari sequensing gen 16S RNA dilanjutkan dengan BLASTN untuk melihat kesamaan di NCBI (National Center for Biotechnology Information). Hasil dari blastn dilanjutkan membuat pohon filogenetik untuk melihat kesamaan dari gen 16S RNA lainnya pada bakteri pada NCBI (National Center for Biotechnology Information). Berdasarkan analisis filogenetik menggunakan gen 16S RNA diperoleh hasil bahwa isolat 1bakteri simbion spons homolog dengan gen 16S RNA pada genus Idiomarina sp sebesar 93 %, sedangkan pada isolat 2 bakteri simbion spons homolog dengan genus Exiguobacterium sebesar 99 %.

Item Type: Thesis (Undergraduate)
Contributors:
ContributionNameNIDN/NIDK
AuthorGultom, Endang SulistyariniUNSPECIFIED
UNSPECIFIEDUNSPECIFIED1981051552009122004
Subjects: Q Science > QR Microbiology
Q Science > QR Microbiology > QR75 Bacteria
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: Mrs Yuni Chairani
Date Deposited: 06 Mar 2018 08:31
URI: http://digilib.unimed.ac.id/id/eprint/29435

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