BIOACTIVE COMPOUNDS IN BUASBUAS (PREMNA PUBESCENS. BLUME) SHOOTS WITH PROTEOMIC APPROACHES USING GC-MS

Buasbuas (Premna pubescens, Blume) is one the most widely used plant in the Melayu traditional food and medicine. Several pharmacological activities including antioxidant effects and phytochemical investigations have been previously reported for the various parts of plant tissues. In the present study, the bioactive compound active compound of the leaf tissues were investigated. Bioactve compound from leaf was monitored using GC-MS investigation. A Gas chromatography-mass spectrometry (GC-MS) system, combined with an in vivo assay for detecting active compounds, was developed in order to identify and inventory the major bio active compound chemicals present in Buasbuas (Premna pubescens, Blume). Through the use of comprehensive spectroscopy studies, the isolated bio active principle was identified. Bio active coumpounds, which was able account for most of the reported biological activity on P. pubescens.