KAJIAN PEMANFAATAN ENZIM BROMEILIN DARI LIMBAH KULIT NANAS (ANANAS COMOSUS (L.) MERR) UNTUK MELUNAKKAN DAGING

Susanti, Dewi (2012) KAJIAN PEMANFAATAN ENZIM BROMEILIN DARI LIMBAH KULIT NANAS (ANANAS COMOSUS (L.) MERR) UNTUK MELUNAKKAN DAGING. Undergraduate thesis, UNIMED.

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Abstract

Bromelin merupakan enzim proteolitik yang terdapat pada tanaman nanas (Ananas comosus (L) Merr).Telah dilakukan isolasi dan penentuan aktivitas spesifik enzim bromelin dari limbah kulit nanas. Pada penelitian ini bromelin diisolasi dengan metoda Dekantasi, lalu ditentukan aktivitas spesifik, uji tekstur dan penentuan kadar protein dengan metoda Lowry menggunakan spektrofotometer serta rancangan penelitian menggunakan Rancangan Acak Lengkap dengan uji ANOVA satu jalur. Hasil isolasi merupakan ekstrak kasar enzim bromelin dengan aktivitas spesifik 16,666x10-3 unit/mg dan pada penelitian diperoleh kondisi optimum pH 6,0 dengan aktivitas spesifik 6,1390x10-3 unit/mg dan tekstur sebesar 11,6280 g/mm3, dan temperatur optimum 55oC dengan aktivitas spesifik 5,2621x10-3 unit/mg dan tekstur sebesar 13,1582 g/mm3, dan konsentrasi enzim optimum sebesar 0,5 gram dengan aktivitas spesifik 6,1461x10-3 unit/mg dan tekstur sebesar 12,959 g/mm3 sementara konsentrasi substrat optimum sebesar 0,75 gram dengan aktivitas spesifik 6,3215x10-3 unit/mm3 dan tekstur sebesar 13,4471 g/mm3

Item Type: Thesis (Undergraduate)
Contributors:
ContributionNameNIDN/NIDK
Thesis advisorSilaban, Ramlan196006181987031002
Call Number: 540 Sus k
Keywords: Bromelin; Aktivitas Spesifik; Tekstur; RAL
Subjects: L Education > LB Theory and practice of education
Q Science > QD Chemistry
Depositing User: Mr Muhammad Iqbal
Date Deposited: 08 Apr 2016 08:34
URI: http://digilib.unimed.ac.id/id/eprint/10134

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