PENGEMBANGAN METODE ANALISIS SPEKTROFOTOMETRY MELALUI REAKSI ENZIMASI UNTUK PENENTUAN GLUKOSA DI DALAM BUAH-BUAHAN

Situmorang, Manihar (2010) PENGEMBANGAN METODE ANALISIS SPEKTROFOTOMETRY MELALUI REAKSI ENZIMASI UNTUK PENENTUAN GLUKOSA DI DALAM BUAH-BUAHAN. Jurnal Sains Indonesia, 34 (02). pp. 49-54. ISSN 1978-3841

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Abstract

The development of spectrophotometric analytical method using enzymatic reaction for the determination of glucose in fruit samples is explained. The study was conducted by integrating of enzyme glucose oxidase (GOx) to oxidize glucose to produce gluconic acid and hydrogen peroxide (H2O2), where the H2O2 under the enzymatic catalysis of peroxidase (POx) was then reacted with o-dianicidine to produce red color of reduced o-dianicidine which was detected spectrophotometrically at 520 nm. The developed method has been applied for the determination of glucose in fruit samples, and the results obtained are compared with biosensor analytical method. The concentration of glucose in fruit samples were found between 0.33-5.71 mg glucose/g samples.

Item Type: Article
Keywords: UV-Vis Spectrophotometry; glucose; o-dianicidine; fruit samples
Subjects: S Agriculture > SB Plant culture
T Technology > T Technology (General)
Divisions: Program Pasca Sarjana > Pendidikan Kimia
Depositing User: Mrs Yuni Chairani
Date Deposited: 05 Apr 2016 02:54
URI: http://digilib.unimed.ac.id/id/eprint/772

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