PHYTOCHEMICAL, ANTIBACTERIAL, ANTIOXIDANT AND ANTICANCER ACTIVITY STUDY OF M. candidum LEAF ACETONE EXTRACT

M. Candidum has been frequently used as a traditional medicine to treat various diseases such as diarrhea,
dysentery, haemorrhoids, cuts and wounds, toothache, and stomach ache. This research was aimed to identify the
activity of M. candidum acetone extract as an antibacterial, antioxidant, anticancer and phytochemical. Antibacterial
activity test was performed in vitro against each of the two Gram-positive and Gram-negative bacteria by paper disc
diffusion method followed by determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. The antioxidant activity of the extract was tested against 2,2-diphenyl1-picrylhydrazyl (DPPH), while the cytotoxic activity of the extract was evaluated against MCF-7 cells. Furthermore, the identification of secondary metabolite content was determined by 1H-NMR spectroscopy. Activity test results
revealed that acetone extract of M.Candidum leaf was activite against four pathogenic bacteria, such as P. acne
ATCC (27853), S. saprophyticus ATCC (49907), S. Mutans ATCV (35668), C. freundii ATCC 8090) with inhibition diameter of 5.70 ± 0.17 - 11.23 ± 0.23 with MIC values of 1250 - 2500 μg /mL and MBC between 1250 - > 5000 μg /mL. M.candidum acetone extract has antioxidant and cytotoxic activity with IC50 value = 22.4761 µg / mL and IC50 = 601.09 ug / mL respectively. Also, the results of phytochemical tests indicated that M. candidum acetone extract contained terpenoids and aromatic compounds.