IDENTIFIKASI MOLEKULER JAMUR ENDOFIT PADA DAUN BUAS-BUAS (Premna pubescens Blume) BERDASARKAN rDNA ITS (INTERNAL TRANSCRIBED SPACER) SEBAGAI ANTIBAKTERI TERHADAP BAKTERI Salmonella thypi DAN Pseudomonas aeruginosa

Safirah, Bella (2021) IDENTIFIKASI MOLEKULER JAMUR ENDOFIT PADA DAUN BUAS-BUAS (Premna pubescens Blume) BERDASARKAN rDNA ITS (INTERNAL TRANSCRIBED SPACER) SEBAGAI ANTIBAKTERI TERHADAP BAKTERI Salmonella thypi DAN Pseudomonas aeruginosa. Undergraduate thesis, UNIMED.

[img] Text
1. NIM 4163220005 COVER.pdf - Published Version

Download (0B)
[img] Text
2. NIM 4163220005 APPROVAL SHEET.pdf - Published Version

Download (0B)
[img] Text
3. NIM 4163220005 ABSTRACT.pdf - Published Version

Download (0B)
[img] Text
4. NIM 4163220005 PREFACE.pdf - Published Version

Download (0B)
[img] Text
5. NIM 4163220005 TABLE OF CONTENT.pdf - Published Version

Download (0B)
[img] Text
9. NIM 4163220005 CHAPTER I.pdf - Published Version

Download (0B)
[img] Text
13. NIM 4163220005 CHAPTER V.pdf - Published Version

Download (0B)
[img] Text
14. NIM 4163220005 BIBLIOGRAPHY.pdf - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg - Published Version

Download (0B)
[img] Other (Generate index codes conversion from text to indexcodes)
indexcodes.txt - Published Version

Download (0B)
[img] Other (Generate index codes conversion from text to indexcodes)
indexcodes.txt - Published Version

Download (0B)
[img] Other (Generate index codes conversion from text to indexcodes)
indexcodes.txt - Published Version

Download (0B)
[img] Other (Generate index codes conversion from text to indexcodes)
indexcodes.txt - Published Version

Download (0B)
[img] Other (Generate index codes conversion from text to indexcodes)
indexcodes.txt - Published Version

Download (0B)
[img] Other (Generate index codes conversion from text to indexcodes)
indexcodes.txt - Published Version

Download (0B)
[img] Other (Generate index codes conversion from text to indexcodes)
indexcodes.txt - Published Version

Download (0B)
[img] Other (Generate index codes conversion from text to indexcodes)
indexcodes.txt - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_lightbox)
lightbox.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_preview)
preview.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_medium)
medium.jpg - Published Version

Download (0B)
[img] Other (Thumbnails conversion from text to thumbnail_small)
small.jpg - Published Version

Download (0B)

Abstract

Identifikasi jamur dengan menentukan taksonominya biasanya didasarkan perbandingan morfologi yang paling menonjol. Identifikasi morfologi tersebut belum memberikan kepastian spesies maupun hubungan kekerabatan dengan spesies. salah satu cara identifikasi yang lebih tepat berdasarkan sekuens DNA ribosomal (rDNA) pada daerah ITS (Internal Transcribed Spacer). Identifikasi secara molekuler berdasarkan urutan DNA ribosomal pada daerah ITS (Internal Transcribe Spacer) merupakan salah satu usaha untuk mengidentifikasi dan membuka informasi genetik suatu jamur endofit. Isolat jamur endofit daun buas-buas (Premna pubescens, Blume) diuji untuk melihat potensi antibakteri terhadap bakteri Salmonella typhi dan Pseudomonas aeruginosa untuk mengetahui potensi antibakterinya kemudian isolat jamur endofit yang memiliki potensi sebagai antibakteri akan diisolasi DNA nya selanjutnya akan dilakukan amplifikasi DNA dengan daerah ITS menggunakan pasangan primer ITS1 dan ITS4. Pada hasil pengamatan makroskopis, mikroskopis, dan identifikasi molekuler dengan mengisolasi DNA dan dianalisis dengan BLAST, dan pohon filogenetik jamur endofit yang ditemukan tergolong dalam genus Aspergillus. Isolasi Jamur endofit dari daun buas-buas memperoleh delapan isolat jamur endofit. Kedelapan isolat jamur endofit tersebut memiliki potensial sebagai antibakteri dengan diameter daya hambat yang berbeda-beda terhadap bakteri uji. pengamatan molekuler dengan menggunakan penanda ITS (Internal Transcribed Spacer) dengan primer ITS1 dan ITS4 teridentifikasi bahwa IJT2 memiliki kekerabatan dengan Genus Aspergillus namun lebih dekat dengan Aspergillus neoellipticus ATCC 16903 dengan persentase kemiripan 100%.

Item Type: Thesis (Undergraduate)
Contributors:
ContributionNameNIDN/NIDK
Thesis advisorRestuati, Martina196303211988032002
Call Number: SK-2021 BIO 071
Keywords: Identifikasi molekuler; Jamur endofit; Salmonella thypi; Pseudomonas aeruginosa; Antibakteri; Daun buas-buas
Subjects: Q Science > QK Botany
Q Science > QK Botany > QK504 Cryptogams
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: Mrs Harly Christy Siagian
Date Deposited: 06 Dec 2022 03:39
URI: http://digilib.unimed.ac.id/id/eprint/49422

Actions (login required)

View Item View Item