PENGEMBANGAN BIOSENSOR SEBAGAI INSTRUMEN ANALISIS UNTUK PENENTUAN KOLESTEROL DI DALAM MAKANAN TRADISIONAL

Situmorang, Manihar and Silitonga, Pasar M. and Nurwahyuni, Isnaini (2012) PENGEMBANGAN BIOSENSOR SEBAGAI INSTRUMEN ANALISIS UNTUK PENENTUAN KOLESTEROL DI DALAM MAKANAN TRADISIONAL. In: Seminar & Rapat Tahunan BKS-PTN B MIPA tahun 2012, 11-12 May 2012, Medan.

[img]
Preview
Text
Cover, Daftar Isi.pdf - Published Version

Download (934kB) | Preview
[img]
Preview
Text
Fulltext.pdf - Published Version

Download (208kB) | Preview

Abstract

The development of a biosensor as instrumental analysis for the determination of cholesterol in traditional food is explained. The biosensor is constructed by immobilizing of enzyme cholesterol oxidase (COx) in deposited polytiramine (Pty) that is attach onto platinized glassy carbon electrode (GC/PT). Cholesterol biosensor (GC/Pt/Pty/COx) developed in this study gave sensitive response to cholesterol, where the detection linearity lies between 0.01–1.0 mM cholesterol, slope 0.180 μA/mM cholesterol, and the detection limit of 0.01 mM cholesterol. The biosensor has been applied for the determination of cholesterol in various types of North Sumatera traditional foods. The contents of the cholesterol in food samples are lies between 0.051-0.835 mM cholesterol. Cholesterol analysis with biosensor has agreed with standard spectrophotometric method for cholesterol (R2 = 0.979).

Item Type: Conference or Workshop Item (Paper)
Call Number: 611 Sit p
Keywords: Biosensor; Polytyramin; Enzyme, Kolesterol; Makanan tradisional
Subjects: Q Science > QP Physiology
R Medicine > R Medicine (General) > R856 Biomedical engineering. Electronics. Instrumentation
R Medicine > RA Public aspects of medicine > RA601 Food and food supply in relation to public health
T Technology > TX Home economics > TX341 Nutrition. Foods and food supply
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia
Depositing User: Mrs Harly Christy Siagian
Date Deposited: 24 May 2016 09:05
URI: http://digilib.unimed.ac.id/id/eprint/4702

Actions (login required)

View Item View Item