UJI KANDUNGAN Coliform DAN Escherichia coli PADA AIR SUMUR GALI DESA SIDO MAKMUR KECAMATAN KUALA KABUPATEN LANGKAT

Wulandari, (2020) UJI KANDUNGAN Coliform DAN Escherichia coli PADA AIR SUMUR GALI DESA SIDO MAKMUR KECAMATAN KUALA KABUPATEN LANGKAT. Undergraduate thesis, UNIMED.

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Abstract

Air sumur gali merupakan sumber air bersih yang digunakan oleh 99,39% warga Desa Sido Makmur Kecamatan Kuala Kabupaten Langkat untuk memenuhi kebutuhan sehari-hari. Mayoritas masyarakat yang memiliki kandang sapi di sekitar sumur gali memicu pentingnya melakukan penelitian kualitas mikrobiologi air, mengingat keberadaan sumur gali yang sangat rentan dengan kontaminasi kotoran sapi. Maka dari itu sampel penelitian diambil berdasarkan jarak sumur gali dengan kandang ternak yang kurang dari 5 meter. Lima sampel yang berasal dari Dusun Inpres, Dusun Petak dua, Dusun Handayani, Dusun Sidorejo dan Dusun Mandailing diuji menggunakan metode Most Probable Number (MPN) di Laboratorium Kesehatan Provinsi Sumatera Utara, untuk mengetahui keberadaan Coliform dan Escherichia coli melalui tahap uji pendugaan, uji penegasan, dan uji kelengkapan serta pewarnaan Gram. Kemudian dilakukan uji IMViC untuk memastikan keberadaan Escherichia coli. Hasil penelitian menunjukkan bahwa semua sampel air sumur gali dengan kode sampel ASG 01, ASG 02, ASG 03, ASG 04 dan ASG 05 positif tercemar Coliform melalui uji penegasan dengan total Coliform yang melewati ambang batas sebanyak 80% dan di bawah ambang batas sebanyak 20% berdasarkan Peraturan Menteri Kesehatan No. 416/MENKES/PER/ IX/1990 tentang Syarat-syarat dan Pengawasan Kualitas Air. Kemudian juga terdapat kandungan Escherichia coli pada kelima sampel tersebut yang melebihi ambang batas yang ditetapkan, hasil positif E.coli juga telah diperkuat melalui hasil uji pewarnaan Gram dan uji IMViC. Maka dari itu sampel air sumur gali yang diuji tidak layak atau tidak memenuhi pesyaratan kualitas mikrobiologi air bersih.

Item Type: Thesis (Undergraduate)
Contributors:
ContributionNameNIDN/NIDK
Thesis advisorNasution, Mhd. Yusuf196312091989031005
Call Number: SK-2020 BIO 076
Keywords: Air sumur gali; Most probable number; Coliform, Escherichia coli; Mikrobiologi air; Bakteri; Air bersih
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Q Science > QR Microbiology > QR75 Bacteria
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: Mrs Harly Christy Siagian
Date Deposited: 27 Oct 2021 02:30
URI: http://digilib.unimed.ac.id/id/eprint/43530

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