Derivatisation of 2,4 (dinitrophenyl hydrazine) DNPH in Canola oil oxidation

Damanik, Marini (2019) Derivatisation of 2,4 (dinitrophenyl hydrazine) DNPH in Canola oil oxidation. Indonesian Journal of Chemical Science and Technology State University of Medan, 02 (1). pp. 80-83. ISSN 2622-4968

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Abstract

Lipid oxidation during heating started at the allylic position of the double bond of unsaturated fatty acids. Primary lipid oxidation which was produced during heating are generally unstable and directly decompose into secondary oxidation products. The oxidative and thermal stability of Rapeseed oil was evaluated using Rancimat 679. Oil samples were heated at 120oC for 10 hours which are taken started from 5, 6, 6.5, 7, 7.5, 8, 9 and 10 hours. Derivatization with DNPH eluted with a mixture of methanol (60%) and water (40%). The aldehydes are analysed by High Performance Liquid Chromatography- Diode Array Detection (HPLC-DAD) after derivatisation with DNPH and identified by high-resolution MS using an orbitrap-based mass spectrometer. HPLC is the suitable method to investigate the non-volatile aldehydes formed by decomposition of hydroperoxides. The result of this oxidation process were described that the maximum point of the oil oxidation was at 9 hours which peak area 378. For oxidation process at the 10 hours the area peak is decreased which point 344.

Item Type: Article
Contributors:
ContributionNameNIDN/NIDK
EditorSimorangkir, Murniatynidn0004055909
EditorSilaban, Ramlannidn0018066008
Keywords: lipid oxidation
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP670 Oils, fats, and waxes
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia
Depositing User: Mrs Elsya Fitri Utami
Date Deposited: 20 May 2020 12:22
URI: http://digilib.unimed.ac.id/id/eprint/39280

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