Harahap, Fauziyah and Diningrat, Diky Setya and Poerwanto, Roedhy and Nasution, Nanda Eska Anugrah and Hasibuan, Rifa Fadhilah Munifah (2019) IN VITRO CALLUS INDUCTION ON SIPAHUTAR PINEAPPLE (Ananas comosus L.) FROM NORTH SUMATRA INDONESIA. Pakistan Journal of Biological Sciences, 22 (11). pp. 518-526. ISSN 1028-8880

Reviewer.pdf - Published Version

Download (201kB) | Preview
Turnitin.pdf - Published Version

Download (3MB) | Preview
Submit accepted.pdf - Published Version

Download (6MB) | Preview
Official URL:


Background and Objective: Sipahutar pineapple (Ananas comosus L.) is a indigenous of pineapple grown in Sipahutar district, North Sumatra, Indonesia. Propagation of Sipahutar pineapple that being done traditionally is less effective, because the number of seeds that produced is very limited and requires a long time. Propagation through in vitro culture is an alternative solution to solve this problem. It is necessary to add plant growth regulator (PGR) to stimulate callus formation in Sipahutar pineapple explants (Ananas comosus L.). Callus induction of pineapple from Sipahutar was carried out by PGR treatment on MS medium. The purpose of this study was to determine the effect MS medium treatment with added dichlorophenoxyacetic acid (2,4-D) and benzyl amino purin (BAP) PGR on Sipahutar pineapple callus formation (Ananas comosus L.) with light and dark treatment. Materials and Methods: This callus induction research used a completely randomized design (CRD) with 2 factors, the first factor was treatment 2,4-D (0, 1, 2) ppm. The second factor is BAP (0, 0.5, 1) ppm. Results: Nine combinations of treatments are obtained. Each combination of treatments is treated in both light and dark conditions. The parameters of this study were the percentage (%) of explants that formed callus, the time of callus formed, callus texture, callus biomass, callus surface height and callus surface area. Data were analyzed with two-way ANOVA, followed by Duncan Multiple Rate Test (DMRT). Conclusion: The study showed that the interaction between 2,4-D and BAP significantly affected the time of callus formed but 2,4-D and BAP did not significantly affect callus biomass, callus surface height and callus surface area. All explants can form callus, except explants without the addition of 2,4-D and BAP. The callus formed on 10 days after induction (DAI) and 12 DAI with the treatment of light and dark. The color of the produced callus were white, yellowish white, greenish white, brown, brownish yellow, brownish white, brownish green, yellowish green and greenish white. The callus formed is generally compact textures, except for explants which by giving 1 ppm 2,4-D produce friable callus.

Item Type: Article
Keywords: Sipahutar pineapple; Callus induction; In vitro culture
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
Q Science > QK Botany > QK640 Plant anatomy
Q Science > QK Botany > QK710 Plant physiology
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: Mrs Harly Christy Siagian
Date Deposited: 12 Feb 2020 02:23

Actions (login required)

View Item View Item