GENETIC VARIABILITY AND CLASSIFICATION OF GANDARIA (Bouea) IN INDONESIA BASED ON INTER SIMPLE SEQUENCE REPEAT (ISSR) MARKERS

The genus Bouea is a member of the Anacardiaceae family whichis widespread in the Malesian Region. This genus consists of two species, namely Bouea oppositifolia (Roxb.) Adelb. and Bouea macrophylla Griffit. This study aims to analyze the genetic diversity Bouea in Indonesia. A total of 75 accessions of B. macrophylla and 30 accessions of B. oppositifolia were analyzed using inter simple sequence repeat (ISSR) markers. The results on B. macrophylla showed that the species were divided into three clusters with a similarity coefficient of 0.35. Group I consists of 53 accessions from Ambon, South Kalimantan, West Kalimantan, Banten, Bogor (Loji, Pandeglag, Leuwisadeng and Jasinga), Cibinong, and Bogor Botanical Garden, whereas Group II consists of 17 accessions from Riau (Batu Sangkar), West Sumatra, Cibinong, Aceh, Medan, Jambi, Palembang, Lampung, and Bangka Belitung. Group III consists of 5 accessions from west Sumatra Province (Batu Sangkar), Bogor Botanical Garden, Jambi, and South Kalimantan. B. oppositifolia is
divided into four groups with a similarity coefficient of 0.84. Group I consists of one accession from Bogor Botanical Garden, Group II consists of 25 accessions from
North Sumatera, Bogor Botanical Garden and Bangka Belitung, Group III consists of two accessions from North Sumatra, whereas group IV consists of two accessions from the Bogor Botanical Garden. The ISSR marker could separate B. macrophylla and B. oppositifolia with a similarity coefficient of 0.34. It was determined that B.
macrophylla and B. oppositifolia were ancestors based on ISSR markers.