EFFECT OF LEAF EXTRACT BUASBUAS (Premna pubescens Blume) FOR AGAINST OF BACTERIA GROWTH Staphylococcus aureus AND Salmonela sp IN VITRO

Restuati, Martina and Pratiwi, Nanda and Widiyastuti, Gita (2017) EFFECT OF LEAF EXTRACT BUASBUAS (Premna pubescens Blume) FOR AGAINST OF BACTERIA GROWTH Staphylococcus aureus AND Salmonela sp IN VITRO. In: The 4th International Conference on Research, Implementation and Education of Mathematics and Science (ICRIEMS), 15-16 Aug 2017, Yogyakarta.

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Official URL: http://aip.scitation.org/doi/pdf/10.1063/1.4995199

Abstract

Buasbuas (Premna pubescens Blume) is the one of the medicinal plants in Indonesia. This plant contains flavonoids potential as an antibacterial. Staphylococcus aureus and Salmonella sp is the one of the bacteria that can cause poisoning. This research aims to find out of inhibitory the extract of buasbuas leaves the growth of Staphylococcus aureus and Salmonella sp. This research was conducted in Medan Health Laboratory used hole sinks methods. Buasbuas leaves which has been condensed ethanol extract was diluted using aquadest with a concentration of 0%, 50%, 60%, 70%, 80%, 90% and dropped into MSA (Mannitol Salt Agar) media inoculated bacteria and wich is a hole sinks. Them incubated for 1 × 24 hours at a temperature of 37°C. The results of this research that the buasbuas extract with the different concentrations can inhibit the growth of Staphylococcus aureus, then the result were analyzed used of Non Factorial Experimental Method (ANOVA) where Fcount (337.54)> Ftable 0,05 (2,77); Ftabel 0.01 (4.25). While the maximum concentration buasbuas extract were more effective the growh of bacteria is 90% with average inhibition zone diameter of 11 mm.

Item Type: Conference or Workshop Item (Paper)
Keywords: Daun buas-buas; Bakteri; Tumbuhan; Staphylococcus aureus; Salmonela
Subjects: Q Science > QK Botany > QK640 Plant anatomy
Q Science > QK Botany > QK710 Plant physiology
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: Mrs Harly Christy Siagian
Date Deposited: 31 Aug 2017 08:15
URI: http://digilib.unimed.ac.id/id/eprint/25991

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