PENGARUH EKSTRAK DAUN BUASBUAS (Premna pubescens Blume.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus SECARA IN VITRO

Widiyastuti, Gita (2017) PENGARUH EKSTRAK DAUN BUASBUAS (Premna pubescens Blume.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus SECARA IN VITRO. Undergraduate thesis, UNIMED.

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Abstract

Buasbuas (Premna pubescens Blume) merupakan salah satu tumbuhan obat di Indonesia. Tumbuhan ini mengandung flavonoid yang berpotensi sebagai antibakteri. Staphylococcus aureus merupakan salah satu bakteri yang dapat menyebabkan keracunan. Penelitian ini bertujuan untuk mengetahui apakah ekstrak daun buasbuas dapat menghambat pertumbuhan bakteri Staphylococcus aureus dan untuk mendapatkan data konsentrasi eksrak daun buasbuas yang menghasilkan zona hambat terbesar. Penelitian ini dilaksanakan di Laboratorium Kesehatan Medan dengan menggunakan metode sumuran. Daun buasbuas yang telah menjadi ekstrak etanol, diencerkan menggunakan aquades dengan konsentrasi 0%, 50%, 60%, 70%, 80%, 90% dan diteteskan ke dalam media MSA (Mannitol Salt Agar) yang telah diinokulasi bakteri serta diberi lubang sumuran. Setelah itu diinkubasi selama 1 24 jam dengan suhu 370C. Hasil penelitian yang diperoleh menunjukkan bahwa pemberian ekstrak daun buasbuas dengan konsentrasi yang berbeda-beda dapat menghambat pertumbuhan bakteri Staphylococcus aureus, kemudian data dianalisis menggunakan Analisis Varians Non Faktorial (ANAVA) menunjukkan bahwa nilai Fhitung (337,54) Ftabel 0,05 (2,77), maupun Ftabel 0,01 (4,25). Dengan konsentrasi maksimum ekstrak yang lebih efektif menghambat pertumbuhan bakteri Staphylococcus aureus adalah 90% dengan rata-rata zona hambat 11 mm.

Item Type: Thesis (Undergraduate)
Contributors:
ContributionNameNIDN/NIDK
Thesis advisorRestuati, Martina196303211988032002
Call Number: SK-2017 BIO 045
Keywords: Premna pubescens Blume, Staphylococcus aureus, Zona Hambat, Sumuran
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology > QR75 Bacteria
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: Mr Maknun
Date Deposited: 25 Apr 2017 02:38
URI: http://digilib.unimed.ac.id/id/eprint/24221

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