MANGOSTEEN DNA ANALYSIS (Garcinia Mangostana L.) WITH MOLECULAR MARKERS AFTER GAMMA RAY IRRADIATION TREATMENT

The objective of this study is to analyse the mangosteen’s DNA with a Random Amplified Polymorphic
DNA (RAPD) marker after being treated with a gamma ray irradiation. The mangosteen seed was cultured on 1/2N Murashige and Skoog (MS 1/2N) medium containing a combination of 5 ppm Benzyl Amino Purine (BAP). The seeds were irradiated through 11 levels of gamma ray, namely 0, 5, 10, 15, 20, 25, 30 35, 40, 45, 50 Gy and then regenerated on MS ½ N + 5 ppm BAP. Results indicated that the molecular analysis using RAPD marker showed changes of DNA band on treated plant. All primers produced 100% polymorphism and were able to be differentiated between the control and treated plants. It can be concluded that mangosteen’s DNA traits can be effectively changed with gamma ray irradiation. This study implies that mangosteen buds can be developed as potential mangosteen seedlings. Mutation could be used to step up genetic variability in order to improve the desired mangosteen traits in future work.