MANGOSTEEN DNA ANALYSIS (Garcinia Mangostana L.) WITH MOLECULAR MARKERS AFTER GAMMA RAY IRRADIATION TREATMENT

Harahap, Fauziyah and Jusoff, Kamaruzaman and Poerwanto, Roedhy and Nusyirwan, and Syarifuddin, and Hasruddin, (2013) MANGOSTEEN DNA ANALYSIS (Garcinia Mangostana L.) WITH MOLECULAR MARKERS AFTER GAMMA RAY IRRADIATION TREATMENT. American-Eurasian Journal of Sustainable Agriculture, 07 (02). pp. 37-44. ISSN 1995-0748

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Official URL: http://www.aensiweb.net/AENSIWEB/aejsa/aejsa/2013/...

Abstract

The objective of this study is to analyse the mangosteen’s DNA with a Random Amplified Polymorphic DNA (RAPD) marker after being treated with a gamma ray irradiation. The mangosteen seed was cultured on 1/2N Murashige and Skoog (MS 1/2N) medium containing a combination of 5 ppm Benzyl Amino Purine (BAP). The seeds were irradiated through 11 levels of gamma ray, namely 0, 5, 10, 15, 20, 25, 30 35, 40, 45, 50 Gy and then regenerated on MS ½ N + 5 ppm BAP. Results indicated that the molecular analysis using RAPD marker showed changes of DNA band on treated plant. All primers produced 100% polymorphism and were able to be differentiated between the control and treated plants. It can be concluded that mangosteen’s DNA traits can be effectively changed with gamma ray irradiation. This study implies that mangosteen buds can be developed as potential mangosteen seedlings. Mutation could be used to step up genetic variability in order to improve the desired mangosteen traits in future work.

Item Type: Article
Keywords: Mangosteen; Tissue culture; Gamma ray; Random Amplified Polymorphic DNA
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany > QK710 Plant physiology
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: Mrs Harly Christy Siagian
Date Deposited: 03 Dec 2016 04:07
URI: http://digilib.unimed.ac.id/id/eprint/21884

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