PENGARUH EKSTRAK METABOLIT SEKUNDER JAMUR ENDOFIT TUMBUH COTYLEOBIUM MELANOXYLON DALAM MENGHAMBAT PERTUMBUHAN MIKROBA PATOGEN

Nurhidayah, - (2014) PENGARUH EKSTRAK METABOLIT SEKUNDER JAMUR ENDOFIT TUMBUH COTYLEOBIUM MELANOXYLON DALAM MENGHAMBAT PERTUMBUHAN MIKROBA PATOGEN. Undergraduate thesis, UNIMED.

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Abstract

Sumber baru bahan bioaktif yang akhir-akhir ini banyak dieksplorasi adalah jamur endofit. Hal ini disebabkan karena kemampuan jamur-jamur endofit memproduksi bahan-bahan bioaktif yang potensial untuk dikembangkan menjadi bahan baku obat. Dalam penelitian ini dilakukan percobaan untuk mengisolasi jamur endofit dari batang tumbuhan raru (Cotylelobium melanoxylon) dan kemudian menguji daya aktivitas biokimia. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak metabolit sekunder dari jamur endofit tumbuhan Cotylelobium melanoxylon dalam menghambat pertumbuhan mikroba patogen yang dilaksanakan pada bulan November 2013 di Laboratorium Mikrobiologi FMIPA UNIMED Jl.Williem Iskandar V Medan.Dalam penelitian ini digunakan sampel yaitu ekstrak jamur endofit. Dari percobaan yang dilakukan diperoleh 38 isolat jamur endofit, kemudian diidentifikasi terdapat 10 genus yang sangat berpotensi, lalu diuji kembali dengan melalui skrining terdapat jamur endofit yaitu Rsi 10 dari genus Nigospora sp yang sangat berpotensi dalam menghambat pertumbuhan bakteri Escherichia colidan Staphylococcus aureus. Jamur ini kemudian difermentasi dan mendapatkan hasil berupa ekstrak yang mangandung senyawa metabolit berupa alkaloid dan flavanoid. Ekstrak tesebut dapat menguji aktivitas biokimia mikroba patogen, jenis mikroba patogen : Collectotrichum,Fusarium oxysporum fsp, Candida albicans,dan Sclerotium rolfsii,Hasil dari uji aktivitas biokimia mikroba patogen yang sangat berpotensi dalam menghambat pertumbuhan mikroba patogen dari ekstrak metabolit sekunder jamur endofit terdapat pada jamur Candida albicans dengan diameter zona hambat sebesar 10,3 mm.

Item Type: Thesis (Undergraduate)
Contributors:
ContributionNameNIDN/NIDK
Thesis advisorHasanah, Uswatun196103011988032002
Call Number: 587.2 Nur p
Keywords: Jamur endofit, Cotylelobium melanoxylon, ekstrak metabolit sekunder, mikroba patogen, zona hambat
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany > QK640 Plant anatomy
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
Depositing User: Mrs Desy Sry Yani
Date Deposited: 08 Apr 2016 08:34
URI: http://digilib.unimed.ac.id/id/eprint/12132

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